From a stretch of genomic DNA to be characterized
by schematic segments A–E (1), a series of
overlapping clones is derived (2). In the first
step a radiolabeled clone is hybridized to
genomic DNA (clone 1) from a DNA library. A
probe (probe A) is used to find the adjacent
clone by hybridizing to a new clone (clone 2).
This establishes that clones 1 and 2 overlap.
Similar steps follow. DNA farther away can be
identified until one reaches the clone that contains
the gene of interest. This procedure, called
chromosome (DNA) walking, can start from
several points and proceed in both directions.
Modifications are used to speed up this process
and to cover large stretches of contiguous DNA.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment