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Genome Maps
Sunday, April 12, 2009
Organization of Eukaryotic Genomes
The genomes of higher organisms contain considerably
more DNA than required for genes
since most of their DNA does not have coding
functions.
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2009
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Genome Maps
Physical and genetic gene maps
STS mapping from a clone library
EST mapping
Approach to Genome Analysis
Sizes of genomes and cloning vectors
Range of resolution within the genome
Alignment of overlapping DNA clones
Organization of Eukaryotic Genomes
The components of the nuclear human genome
Satellite DNA
Long interspersed nuclear elements (LINEs)
Short interspersed nuclear elements (SINEs)
Gene Identification
Different approaches to identifying a disease-rela...
Positional cloning
candidate gene
Principal steps in gene identification
ORF
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